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paav cag egfp vector  (Addgene inc)


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    Structured Review

    Addgene inc paav cag egfp vector
    Paav Cag Egfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+cag+egfp+vector/AAV+pCAG-FLEX-EGFP-WPRE+(Plasmid+%2351502)/pm41741636-453-58-60
    Average 96 stars, based on 186 article reviews
    paav cag egfp vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Fluorescence:

    Article Title: CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.
    Article Snippet: .. For fluorescence imaging, mEGFP–PLIN2, mEGFP–CB5 (residues 90 to 124 at the C-terminal end of cytochrome b5 type A, CYB5A), mEGFP–Sec61β, IgH signal peptide–mEGFP–KDEL, APOE–mEGFP, ApoE–GFP1-10 and GFP11–mCLCC1 (GFP11 was inserted into the N terminus of mCLCC1, followed by the signal peptide) were cloned between the KpnI and HindIII restriction sites to substitute for enhanced GFP (eGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1–Flag, twin StrepTagII–TMEM41B and Flag–TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Article Title: Torsins Organize CLCC1 Assembly to Safeguard ER Bilayer and Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). sgRNAs were designed using the Benchling platform ( https://benchling.com/ ) to optimize editing efficiency and minimize the unintended off-target effects. ..

    Article Title: CLCC1 Governs Bilayer Equilibration at the Endoplasmic Reticulum to Maintain Cellular and Systemic Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), mEGFP-Sec61β, IgH signal peptide-mEGFP-KDEL, APOE-mEGFP, ApoE-GFP1-10, and GFP11-mCLCC1 (GFP11 was inserted into the N-terminus of mCLCC1, followed by the signal peptide) were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1-FLAG, twin StrepTagII-TMEM41B and FLAG-TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Imaging:

    Article Title: CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.
    Article Snippet: .. For fluorescence imaging, mEGFP–PLIN2, mEGFP–CB5 (residues 90 to 124 at the C-terminal end of cytochrome b5 type A, CYB5A), mEGFP–Sec61β, IgH signal peptide–mEGFP–KDEL, APOE–mEGFP, ApoE–GFP1-10 and GFP11–mCLCC1 (GFP11 was inserted into the N terminus of mCLCC1, followed by the signal peptide) were cloned between the KpnI and HindIII restriction sites to substitute for enhanced GFP (eGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1–Flag, twin StrepTagII–TMEM41B and Flag–TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Article Title: Torsins Organize CLCC1 Assembly to Safeguard ER Bilayer and Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). sgRNAs were designed using the Benchling platform ( https://benchling.com/ ) to optimize editing efficiency and minimize the unintended off-target effects. ..

    Article Title: CLCC1 Governs Bilayer Equilibration at the Endoplasmic Reticulum to Maintain Cellular and Systemic Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), mEGFP-Sec61β, IgH signal peptide-mEGFP-KDEL, APOE-mEGFP, ApoE-GFP1-10, and GFP11-mCLCC1 (GFP11 was inserted into the N-terminus of mCLCC1, followed by the signal peptide) were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1-FLAG, twin StrepTagII-TMEM41B and FLAG-TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Clone Assay:

    Article Title: CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.
    Article Snippet: .. For fluorescence imaging, mEGFP–PLIN2, mEGFP–CB5 (residues 90 to 124 at the C-terminal end of cytochrome b5 type A, CYB5A), mEGFP–Sec61β, IgH signal peptide–mEGFP–KDEL, APOE–mEGFP, ApoE–GFP1-10 and GFP11–mCLCC1 (GFP11 was inserted into the N terminus of mCLCC1, followed by the signal peptide) were cloned between the KpnI and HindIII restriction sites to substitute for enhanced GFP (eGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1–Flag, twin StrepTagII–TMEM41B and Flag–TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Article Title: Torsins Organize CLCC1 Assembly to Safeguard ER Bilayer and Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). sgRNAs were designed using the Benchling platform ( https://benchling.com/ ) to optimize editing efficiency and minimize the unintended off-target effects. ..

    Article Title: CLCC1 Governs Bilayer Equilibration at the Endoplasmic Reticulum to Maintain Cellular and Systemic Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), mEGFP-Sec61β, IgH signal peptide-mEGFP-KDEL, APOE-mEGFP, ApoE-GFP1-10, and GFP11-mCLCC1 (GFP11 was inserted into the N-terminus of mCLCC1, followed by the signal peptide) were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1-FLAG, twin StrepTagII-TMEM41B and FLAG-TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Plasmid Preparation:

    Article Title: CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assembly.
    Article Snippet: .. For fluorescence imaging, mEGFP–PLIN2, mEGFP–CB5 (residues 90 to 124 at the C-terminal end of cytochrome b5 type A, CYB5A), mEGFP–Sec61β, IgH signal peptide–mEGFP–KDEL, APOE–mEGFP, ApoE–GFP1-10 and GFP11–mCLCC1 (GFP11 was inserted into the N terminus of mCLCC1, followed by the signal peptide) were cloned between the KpnI and HindIII restriction sites to substitute for enhanced GFP (eGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1–Flag, twin StrepTagII–TMEM41B and Flag–TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Article Title: Torsins Organize CLCC1 Assembly to Safeguard ER Bilayer and Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). sgRNAs were designed using the Benchling platform ( https://benchling.com/ ) to optimize editing efficiency and minimize the unintended off-target effects. ..

    Article Title: SINEUP Non-coding RNA Targeting GDNF Rescues Motor Deficits and Neurodegeneration in a Mouse Model of Parkinson’s Disease
    Article Snippet: .. AAV-miniSINEUP-GDNF was generated modifying an existing pAAV-CAG-EGFP vector (Addgene, 28014) by inserting between the intron and the GFP a sequence coding for the miniSINEUP-GDNF , a polyA signal, and a PGK promoter. ..

    Article Title: CLCC1 Governs Bilayer Equilibration at the Endoplasmic Reticulum to Maintain Cellular and Systemic Lipid Homeostasis
    Article Snippet: .. For fluorescence imaging, mEGFP-Perilipin2, mEGFP-CB5 (residues 90 to 124 at the carboxyl-terminal end of cytochrome B5 type A, CYB5A), mEGFP-Sec61β, IgH signal peptide-mEGFP-KDEL, APOE-mEGFP, ApoE-GFP1-10, and GFP11-mCLCC1 (GFP11 was inserted into the N-terminus of mCLCC1, followed by the signal peptide) were cloned between KpnI and HindIII restriction sites to substitute for enhanced GFP (EGFP) in the pAAV-CAG-EGFP vector (Addgene, 51502). .. For protein purification, CLCC1-FLAG, twin StrepTagII-TMEM41B and FLAG-TMEM41B were cloned into pKH3 (Addgene, 12555) between the EcoRI and XbaI restriction sites.

    Bioprocessing:

    Article Title: SINEUP Non-coding RNA Targeting GDNF Rescues Motor Deficits and Neurodegeneration in a Mouse Model of Parkinson’s Disease
    Article Snippet: .. AAV-miniSINEUP-GDNF was generated modifying an existing pAAV-CAG-EGFP vector (Addgene, 28014) by inserting between the intron and the GFP a sequence coding for the miniSINEUP-GDNF , a polyA signal, and a PGK promoter. ..

    Generated:

    Article Title: SINEUP Non-coding RNA Targeting GDNF Rescues Motor Deficits and Neurodegeneration in a Mouse Model of Parkinson’s Disease
    Article Snippet: .. AAV-miniSINEUP-GDNF was generated modifying an existing pAAV-CAG-EGFP vector (Addgene, 28014) by inserting between the intron and the GFP a sequence coding for the miniSINEUP-GDNF , a polyA signal, and a PGK promoter. ..

    Sequencing:

    Article Title: SINEUP Non-coding RNA Targeting GDNF Rescues Motor Deficits and Neurodegeneration in a Mouse Model of Parkinson’s Disease
    Article Snippet: .. AAV-miniSINEUP-GDNF was generated modifying an existing pAAV-CAG-EGFP vector (Addgene, 28014) by inserting between the intron and the GFP a sequence coding for the miniSINEUP-GDNF , a polyA signal, and a PGK promoter. ..

    Article Title: The sperm quality in DIO male mice is linked to the NF-κB signaling and Ppp2ca expression in the hypothalamus
    Article Snippet: .. The coding sequence (CDS) of the constitutively active fragment of IKKβ ( Ikkβ CA) and Ppp2ca was inserted into the pAAV-CAG-EGFP vector (Addgene, catalog No: 37825) ( A) to generate the pAAV-CAG- Ikkβ CA-3×HA and pAAV-CAG-EGFP- Ppp2ca -3×HA ( B and S1C). .. The CDS of Ikkβ CA and Ppp2ca was inserted into the pLV3-CMV-MCS - 3×HA-Puro vector (Miaolingbio, catalog NO: P30780) ( D) to create the pLV3-CMV- IkkβCA -3×HA-Puro and pLV3-CMV- Ppp2ca -3×Flag-Puro ( E and S1F).



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    Image Search Results


    Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections

    doi: 10.1073/pnas.2521091122

    Figure Lengend Snippet: Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.

    Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.

    Techniques:

    Development of eCb–hindbrain projections . 3D visualization of the development of excitatory cerebellar (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) efferent projections innervating the hindbrain. The movie highlights the progressive expansion of eCb axonal pathways across hindbrain regions.

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    Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections

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    Figure Lengend Snippet: Development of eCb–hindbrain projections . 3D visualization of the development of excitatory cerebellar (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) efferent projections innervating the hindbrain. The movie highlights the progressive expansion of eCb axonal pathways across hindbrain regions.

    Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.

    Techniques: